After singing in the choir of the Chapel Royal as a boy, Samuel Sebastian embarked on a career as a musician, and was appointed organist at Hereford Cathedral in 1832. While there he married the sister of the Dean, John Merryweather. He moved to Exeter Cathedral three years later, and subsequently held appointments at Leeds Parish Church (from 1842), Winchester Cathedral (from 1849) and Gloucester Cathedral (1865-1876). In 1839 he received both his Bachelor of Music degree and a Doctorate of Music degree from Oxford. He became a Professor of Organ at the Royal Academy of Music in 1850. He died at his home in Gloucester on 19 April 1876 aged 65. He is buried next to his daughter in St. Bartholomew's Cemetery in Exeter by the old City Wall. There are memorial tablets to him in Exeter Cathedral and Winchester Cathedral, and his memorial at Gloucester Cathedral is in stained glass.