Ayola was born in Cardiff, Wales in May 1968, to a Sierra Leonean mother and a Nigerian father. She was raised by her mother's cousin and his wife in Ely, Cardiff. Ayola's heritage means she is Yoruba by descent, although she does not speak the language. Ayola studied at Windsor Clive Primary and Glan Ely High School, and was a member of the Orbit Youth Theatre, South Glamorgan Youth Theatre, South Glamorgan Youth Choir and the National Youth Theatre of Wales. She left high school before sitting her A Levels in order to pursue her ambition of becoming an Actress. She explains: "I've always wanted to act. I decided at 16 I wanted to make my living acting, but even if I couldn't, I’d be in an amateur theatre company." She then went on to attend the Royal Welsh College of Music and Drama, studying for a three-year acting diploma. Her first acting role was for the Welsh Eisteddfod when still at primary school, playing a lady-in-waiting at the court of King Arthur. Ayola has stated that it was Barbra Streisand's performance in Hello, Dolly! that inspired her to act as a child, though credits her adoptive mother with encouraging her to act professionally. Ayola's first job was selling jeans on Bessemer Road Market in Cardiff. She worked as a chambermaid whilst attending drama school, and, six weeks prior to graduation, was offered a job with the 'Made in Wales' theatre company which enabled her to obtain her union card.
Ayola has undertaken the following roles from her screen and televisual debut in 1993, to the present day.
During a 1998 production of Hamlet, Ayola met her Future husband, fellow actor Adam Smethurst – son of Love Thy Neighbour star Jack Smethurst. They met again two years later, during a production of Twelfth Night, and went on to become a couple. The pair married in May 2004, and Ayola gave birth to their first daughter, Tansy, in July that year. Their second daughter, Shani, was born in January 2009.
Throughout her career, Ayola has been outspoken on the subject of racial discrimination in the entertainment industry. Describing her motivation, she states: "I am not an overtly political person. I just want fairness". Ayola believes that black actors receive less recognition than their white counterparts, explaining; "If you get a show with six stars and one is black you are more likely to see interviews with the five white actors. [...] They are not being sold as a reason to watch." She believes that her career would have taken her in a different direction were she not of ethnic origin, stating: "I could not have played any of the roles I have played on TV if I was white [...] I am very aware of where the glass ceiling is and it's still very low and expectations are still very low". She has noted having casting Directors accept the notion of characters being both black and Welsh to be a particular Problem, explaining that: "I get offered a lot of very different roles, but they're never Welsh. [...] The one time I was asked to play a Welsh character on screen was in Tiger Bay for BBC Wales, but I know if that series had been called Radyr Park or Cyncoed Close I wouldn't have been in it". In 2001, Ayola founded a production company and directed a short film entitled Persephone's Playground. She presented the film at the Cannes film festival, using it as part of her campaign for increased black representation in theatre, films and television. The project, however, was largely unsuccessful, with Ayola stating: "it just made me decide that if there's anything I don't want to do, it's produce films, because I'm rubbish at it. I was so bad with the budget that I just said yes to everything and then had to worry about how to pay for things at the end." In 2008, Ayola offered her support to the Action for Southern Africa campaign Dignity! Period, aiming to provide affordable sanitary protection to Zimbabwean women.
Ayola describes herself as "optimistic, cynical, lazy, naive [and] honest". She spends her free time "watching television, the theatre, cinema, concerts, indoor wall climbing [and] keeping fit [with] yoga [and] aerobics". She has a collection of Troll dolls which she keeps under her bath. Ayola has an interest in travel, and has visited Peru and Kenya among other destinations. She has expressed a Desire to visit Brazil and Argentina, and has "a romantic notion of travelling around Europe in a camper van". Ayola often speaks of her pride in her Welsh upbringing, describing herself as "an Ely girl through and through". She was made a Fellow of the Royal Welsh College of Music and Drama in 2003.
Ayola was nominated and shortlisted for the 'Female Performance in TV' award in the 2006 Screen Nation Awards, for her role as Kyla Tyson in Holby City. She received Honourable Mention for the same role the following year, and was shortlisted again in 2008. In 2005, WalesOnline voted Ayola the 29th sexiest woman in Wales, saying of her: "One of our favourite thesps, she's black, beautiful and the term "yummy mummy" should have been made for her." She placed 40th in 2008, with the Western Mail noting: "the last 15 years have only seen Rakie become more beautiful and more successful". Ayola placed 17th in the awards in 2009.
In October 2008, Ayola announced that she would be departing from Holby City in order to have a second child, stating: "I've had a great time and I'll miss it. I made the decision purely because I was pregnant, but I wasn't really ready to put down the character of Kyla. I'm very sorry to have to let her go."
Ayola's other notable television appearances include the BBC psychological thriller Green-Eyed Monster (2001), soap opera EastEnders (2001), Waking the Dead (2001), London's Burning (2001), Offenders (2002), Murder in Mind (2003), The Canterbury Tales (2003) and Sea of Souls (2004). In 2008, she starred in the Doctor Who episode "Midnight", playing an intergalactic Hostess alongside David Tennant's Tenth Doctor. In 2009, Ayola starred in the CBBC musical comedy My Almost Famous Family. She stated: "The script made me laugh out loud when I read it. [...] I also like the fact that there were a lot of politically-correct boxes being ticked, but the Writers and Producer haven't been restrained by that. "So, instead of bowing to this altar, they've said, 'Okay, we have this family that's half-black, half-white, half-American, half-British. We have a mix of boys and girls, one character who's mixed-raced and deaf – but we're not going to be restrained by any of that. We're not going to tiptoe around Martha's disability or anything.' I liked that. It wasn't some sort of reverential hands-off approach to what we're presenting." She has also been cast in the film Dredd. She will join the cast of Harry Potter and the Cursed Child at the Palace Theatre, London from 24 May 2017, playing the role of Hermione Granger.
