In 1960, the McArdle Laboratory for Cancer Research at the University of Wisconsin - Madison recruited him as a virologist; a position that had been hard to fill because, at the time, virology was not considered pertinent to cancer research. Even though Temin knew he would be completely independent in Madison, because of the lack of research involving virology and oncology together, Temin stated that he was “supremely self-confident.” When he first arrived in Madison in 1960, he found an unprepared laboratory in the basement of a rundown building with an office that could be considered a closet. Until a more suitable laboratory could be prepared, he continued his research with RSV at a friend’s laboratory at the University of Illinois. Later that year, he returned to Madison, continued his RSV research in his own lab, and began his position as an assistant professor.