Lwoff was born in Ainay-le-Château, Allier, in Auvergne, France, the son of Marie (Siminovitch), an Artist, and Solomon Lwoff, a Psychiatrist. He joined the Institute Pasteur in Paris when he was 19 years old. In 1932, he finished his PhD and, with the help of a grant from the Rockefeller Foundation, moved to the Kaiser Wilhelm Institute for Medical Research of Heidelberg to Otto Meyerhof, where he did research on the development of flagellates. Another Rockefeller grant allowed him go to the University of Cambridge in 1937. In 1938, he was appointed departmental head at the Institut Pasteur, where he did groundbreaking research on bacteriophages, microbiota and on the poliovirus.